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      Burkert Contromatic Australia Pty. Limited
      15 Columbia Way
      Norwest Business Park
      Baulkham Hills, NSW 2153

      PO Box 6524
      Baulkham Hills NSW 2153
      Phone +61 1300 888 868
      Fax +61 1300 888 076
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      First Avenue
      Mawson Lakes SA 5095

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      Unit 8, 1st Floor
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      Joondalup WA 6027

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      Unit 5a, 88 Brandl Street
      Brisbane Technology Park
      Eight Mile Plains QLD 4113

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      Suite 49, UniPark
      Building 4, 195 Wellington Road
      Clayton VIC 3168

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      Bürkert Sydney
      15 Columbia Way
      Norwest Business Park
      Baulkham Hills NSW 2153

      E-Mail fnyrf.nh@ohexreg.pbz
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Liquid chromatography

"Chromatography" is an umbrella term covering physical methods in which substances are separated via distribution between a stationary and mobile phase.

HPLC (High-Performance Liquid Chromatography) is a separating process in which a liquid sample is transported through a stationary phase under high pressure. Depending on the nature of the interaction between the phases and the sample, we distinguish between adsorption chromatography, partition chromatography, ion-exchange chromatography, sizeexclusion chromatography and affinity chromatography. The processes of adsorption chromatography and partition chromatography are the chief processes used in HPLC. HPLC uses partition particles with grain sizes of a few µm; it thus achieves high numbers of plates or trays, but at the same time, requires a high counter-pressure to be overcome during transport of the mobile phase through the thin separation column (of a few millimeters diameter). All parts must be interconnected without any dead volume wherever possible and must be pressure-stable (up to 300 bar).